CONFOCALMICROSCOPY Archives

July 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: Depth resolution?
From:
Dr M Cannell <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 16 Jul 1996 16:25:20 PDT
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On Tue, 16 Jul 1996 09:38:14 +0000 Antoine THILL wrote:
 
 
>
> I'm working in reflection mode with a Leica confocal.
> Can someone give a definition of depth resolution and a (simple)
> way to measure it experimentally.
>
 
Dear Antoine:
 
Just record a Z-section with a front surface mirror flat on the
stage as the object. This will give you a quick measure of the
z-resolution.
 
Regards Mark Cannell
 
P.S. Dont forget the coverslip and immersion oil!

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