CONFOCALMICROSCOPY Archives

July 1997

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Subject:
Re: staining biopolymer matrices
From:
Phil Allen <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 25 Jul 1997 10:28:05 -0400
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Stefan

See the paper "Sheils, et al; 1996. Actin mediates DNA fiber formation in
chronic inflammatory airway disease and in vitro: actin depolymerization
dissolves DNA bundles.  Am J. Path.148:919-927."   We stained DNA and actin
filaments in CF and chronic bronchitus sputum using YoYo-1 and rhodamine
phalloidin.  As for the other components, I'm  not aware of specific lipid
or glycoprotein dyes.  Good luck.

Phil Allen

________________
Philip G. Allen; Ph.D.
Instructor in Medicine
Brigham and Women's Hospital
Division of Experimental Medicine
LMRC 301
221 Longwood Ave.
Boston, MA 02115

617-278-0321 (lab)
       -734-2248 (fax)

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