I would like to coat some glass dishes with vitronectin in order to help cells 
spread and image the adhesion structures.

I understand there are several factors that will affect adherance such as 
density of vitronectin used per cm2 etc.

However, how long does this layer last once you have plated your cells out on 
them? 

Hours? Days? Weeks?

Once a cell has moved over a part of vitronectin coating, is that vitronectin 
coat "used"?

My question comes from a basis that using FCS (in which I beleive vitronectin 
is one of the most concentrated adhesive proteins) to coat my dishes only 
seems to help cells spread when it has been newly coated, and not once the 
dish has been in use for 3 days (ie, observation is that precursor cells in 3 day 
samples eventually differentiate but hardly spread. If you take these 
precursors and plate them on new dishes..bingo!!!!!!!!!).

sEB