*****
To join or leave the confocal microscopy listserv or to change your email address, go to:
https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Claire,

I like to use CM-DII. It's PFA fixable, so although it's a lipophilic dye,
it will stay in place even after tissue clearing. I've never tried tail
vein injections, just transcardial. It gives incredible staining, but might
not be that much cheaper than lectin.  See Figure 9 here:
https://pubmed.ncbi.nlm.nih.gov/35128463/

I also have another group that uses fluorescein-labeled-albumin in warm
gelatin. This is probably more economical. the protocol here:
https://www.jneurosci.org/content/29/46/14553

-Doug

On Thu, Mar 10, 2022 at 5:07 PM Claire Brown, Dr. <[log in to unmask]>
wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We are working on staining blood vessels in mouse brain sections using
> tail vein injection of a fluorescent lectin.
> The protocol we have uses a lot of an expensive reagent so we wanted to
> see what reagents people are using.
>
> The protocol we have is working well but the reagent is costly!
>
> Any advice is appreciated.
>
> Sincerely,
>
> Claire
>