Sundar,

unfortunately, over human visual comparison there is no standard technique.
Adding to Louis suggestion, try to use larger fluorescent beads (1-5µm
depending on your objective) that are only stained on the surface. This way
you can see better how well aberrations had been dealt with.

Regards
Lutz

----- Original Message ----- 
From: "sundar" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, September 21, 2009 10:08 AM
Subject: Validating deconvolution


> Hello all,
>
> I'm using a deconvolution software to deconvolve a 3D stack. Does anyone
> know of any way in which I can measure the improvement over the original
> dataset? Are there any standard techniques for the same?
>
> Thanks,
> Sundar
> -- 
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