Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Yes, I forgot about zooming by powers of 2 due to the galvos.  There  
should be a thread in the archives on that.
Our 1024 was mothballed for about 2 years and I just got it going  
again.  I've got much recall, or mistakes to repeat, on that system.

Regards
Glen
On Feb 11, 2008, at 9:50 AM, Martin Wessendorf wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Glen MacDonald wrote:
>
>> Nyquist sampling at 2.3 times the object frequency is adequate for  
>> detection, but to resolve the point to point Rayleigh resolution,  
>> you need to double it again to 4.6X the object frequency,  
>> especially if deconvolving.
>
> I may be wrong on this, but unless they fixed it sometime while my  
> back was turned, the only "true" zooms possible on a BioRad are 1,  
> 2, 4 and 8x.  Intermediate values requires double-scanning some  
> lines.  Using such a value can result in artifacts when deconvolving.
>
> Take care!
>
> Martin Wessendorf
> -- 
> Martin Wessendorf, Ph.D.                   office: (612) 626-0145
> Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
> University of Minnesota             Preferred FAX: (612) 624-8118
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
> Minneapolis, MN  55455
> **MY E-MAIL ADDRESS HAS CHANGED.  PLEASE USE [log in to unmask] **