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September 2014

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Subject:
Drying down Cy3-NHS into aliquots
From:
Christophe Leterrier <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 18 Sep 2014 13:29:53 +0200
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Hi,

A question for the people who make their fluorescent probes:

I have split 1 mg Cy3-NHS into 10 x 0.1 mg using 20 µL DMSO into 10 x 2 µL.
Now I'm trying to evaporate the DMSO but it seems quite difficult (it's
been two hours in the Speedvac/cold trap and it's still not dried). Any
suggestions on how to proceed (or an alternative method to DMSO)?

Thanks for your help,

Christophe

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