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Hi Abby,



For our spinning disk microscope, we've recently purchased the exact
Olympus X-line model you mentioned:

UPLXAPO100XO (N.A 1.45, FN 26.5, coverslip 0.17, WD 0.13mm)

Earlier we were using:

UPLFLN100XO (N.A: 1.3, FN26.5, coverslip 0.17, WD 0.2mm)

(We put the old one back to Olympus Cell-R microscope from which we
borrowed it after the arrival of the new spinning disk microscope)

The old one has a slightly longer working distance (0.2 vs 0.13mm), but the
new X-line objective is clearly brighter and has better resolution (NA 1.45
vs 1.3). In our case, it significantly improved the performance of our
spinning disk microscope.

Regards,

Ferhan


---
Ferhan Ayaydin, Ph.D.
---

*Head of Advanced Core Facility *HCEMM, Hungarian Centre of Excellence for
Molecular Medicine
Functional Cell Biology and Immunology Advanced Core Facility
University of Szeged, Faculty of Medicine, 6720 Szeged, Hungary
---

* Senior research associate *Biological Research Centre, Cellular Imaging
Laboratory
Temesvári krt. 62, 6726 Szeged, Hungary
---

* Research associate *University of Szeged, Faculty of Medicine,
Albert Szent-Györgyi Health Centre
Interdisciplinary R&D and Innovation Center of Excellence
Department of Dermatology and Allergology,
Korányi fasor 6, 6720 Szeged, Hungary



On Thu, May 20, 2021 at 9:30 PM Abby Dernburg <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all -
>
> I am thinking of adding an Olympus X-Line 100X NA 1.45 to our DeltaVision
> Elite system and am curious about real-world experience with this
> objective, particularly for wide-field deconvolution microscopy. I haven't
> found any descirptions of performance other than marketing materials so I'm
> wondering how it measures up. Thanks in advance for any feedback!
>
> -Abby
>