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 Hi Darren,

We have used our Zeiss PALM-Laser capture microdissection microscope to cut
motor neurons of zebrafish embryos.

Best regards,
Esther

*Dr. Esther G.L. Koh* :: Head, Advanced Imaging Laboratory :: Life Sciences
Institute Immunology Programme :: National University of Singapore :: Centre
for Life Sciences, 28 Medical Drive #03-06E, Singapore 117456

On Thu, 6 May 2021 at 20:37, Straatman, Kees (Dr.) <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Darren,
>
> I have had users using the 405 nm laser on a CLSM for ablation; see for
> example https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331284/
>
> Best wishes
>
> Kees
>
> Dr Ir K.R. Straatman FRMS
> Advanced Imaging Facility
> University of Leicester
> www.le.ac.uk/advanced-imaging-facility
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[log in to unmask]> On
> Behalf Of Darren Robinson
> Sent: 06 May 2021 08:52
> To: [log in to unmask]
> Subject: Lasers for ablation of biological samples
>
> *****
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>
> Hi,
>
> We currently use a nitrogen laser ablation system (Micropoint from Andor)
> to perform wounding studies on drosophila embryos.  The laser is capable of
> reliably inducing a local injury to superficial tissue that is visible when
> the laser is fired.  Does anyone have any experience using other lasers for
> this kind of work?  Would a high power 405nm laser work for example, or
> does ablation require using the lower wavelength pulsed light that is
> produced by lasers such as the one we currently use?
>
> Many thanks,
>
> Darren.
>


-- 
Dr. Esther G.L. Koh :: Head, Advanced Imaging Laboratory :: Life Sciences
Institute Immunology Programme :: National University of
Singapore :: Centre for Life Sciences, 28 Medical Drive #03-06E, Singapore
117456