Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear Sophie et al,
we have made excellent experience using a Zeiss PlanApo 60x W NA 1.2 lense
without coverglass correction in combination with BioFolie (see link below),
which has a refractive index of 1.35. There is a hydrophilic and a hydrophobic
modification available.
http://www.ivss.de/en/Products/Culture_Systems/bioFOLIE_25___petriPERM/body_biofolie_25___petriperm.html
The main advantage for us was that this transparent film is 25micron
thin which
increased the usable working distance of the objective by roughly
150micron, but
there is also thicker and more stable variants of it.
No commercial interest whatsoever,
with best regards,
jens
Quoting Beat Ludin <[log in to unmask]>:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal
>
> Cytop appears to be a resin meant mainly for coating. So you might have to=
> =20
> mold your own coverslips. And since it is a perfluorinated polymer, you=20
> would probably have to activate the surface in case you want to have cells=
> =20
> attach to it. This might be difficult because it is chemically inert and=20
> both hydrophobic and lipophobic at the same time. I have a suspicion that=20
> it wasn't the lack of demand that made Olympus give up on it. I'm not a=20
> chemist, though, and I can only encourage you to give it a try.
>
> Beat
>
> At 08:42 02-09-2005, you wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal
>>
>> Dear Peter and Sophie,
>>
>> Asahi Glass Company is the company that Olympus was cooperating with to=20
>> develop the Cytop coverslip. I guess if you can get a thin piece of cytop=
> =20
>> plastic from Asahi, then it should do the trick. Let us know if you=20
>> succeed and what it ends up costing.
>>
>> Cheers
>>
>> Stephen H. Cody
>>
>> Microscopy Manager
>> Central Resource for Advanced Microscopy
>> Ludwig Institute For Cancer Research
>> PO Box 2008 Royal Melbourne Hospital
>> Parkville Victoria 3050
>> Australia
>> Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
>> email: [log in to unmask]
>> www.ludwig.edu.au/labs/confocal.html
>> www.ludwig.edu.au/confocal
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[log in to unmask]] On=20
>> Behalf Of Peter Wall=E9n
>> Sent: Friday, 2 September 2005 4:25 PM
>> To: [log in to unmask]
>> Subject: Re: Cytop coverslips
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal
>>
>> Dear Sophie,
>>
>> I don=B4t know if Asahi Glass Company makes CYTOP coverslips, but they
>> have a website about the product:
>>
>> http://www.agc.co.jp/english/chemicals/shinsei/cytop/index.html
>>
>> I hope it may be of some help.
>>
>> Best regards,
>>
>> Peter W
>>
>>
>> S. Brunet wrote:
>> > Search the CONFOCAL archive at
>> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal
>> >
>> > Hello,
>> >
>> > I would like to know a supplier of Cytop coverslips.
>> >
>> > We have a Water Immersion objective (no coverslip). From reading the=20
>> archives,
>> > am I correct in saying that this objective CAN be used with a Cytop=20
>> coverslip?
>> > What thickness coverslip is suitable? (I am getting the impression that=
> =20
>> it does
>> > not matter).
>> >
>> > Is it ok to glue those to the bottom of Petrie Dishes?
>> >
>> > Thank you,
>> > Sophie
>> > _______________________________________
>> > Sophie M.K. Brunet, Ph.D.
>> > Research Officer
>> > Optical Spectroscopist, Laser Systems and Applications
>> > [log in to unmask]
>> > 306-966-1719 (office) 306-966-1702 (fax)
>> > _______________________________________
>> > Saskatchewan Structural Sciences Centre
>> > University of Saskatchewan
>> > Thorvaldson Bldg.
>> > 110 Science Place
>> > Saskatoon, SK S7N 5C9
>> >
>>
>> --
>> ****************************
>> Peter Wall=E9n
>> Ph.D., Assoc. Prof.
>>
>>
>>
>> Nobel Institute for Neurophysiology,
>> Department of Neuroscience,
>>
>> Karolinska Institute
>>
>> Retzius v=E4g 8, A3:5
>> SE-171 77 Stockholm, Sweden
>>
>>
>>
>> Phone: +46-8-524 86913
>>
>> Fax: +46-8-34 95 44
>> ****************************
>
--
Dr. Jens Rietdorf
EMBL, Meyerhofstr.1,
D-69117 Heidelberg,
Cell Biology/Biophysics Program,
Advanced Light Microscopy Facility
Phone ++49(0)6221 387-467 FAX-306
http://www.embl.de/almf
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