CONFOCALMICROSCOPY Archives

January 1996

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Subject:
Zeiss LSM410: FRAP macros?
From:
Harold Ross Payne <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 31 Jan 1996 10:41:37 -0500
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Hello confocal list readers:
 
  I would like some help from microscopists that have used a confocal
microscope, particularly the Zeiss LSM410, for the technique of
fluorescence recovery after photobleaching (FRAP).
 
  What are the best ways to manipulate the variables: spot size, energy,
time, monitor recovery?  I have been recording macros to remove
attenuation, bleach with a spot scan for ? time and monitor recovery by a
time series of an roi.
 
  Could anyone please help me with examples of macros that they have written?
 
Thanks,
Ross
 
Harold Ross Payne
Department of Neurosciences
2109 Adelbert Rd.
Case Western Reserve University
Cleveland, Ohio 44106
 
Internet: [log in to unmask]
Phone: (216)368-2575
Fax: (216)368-4650

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