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Hello Carl,
I have a new position at the university, and shut down that server.
About finishing the project...
Well the student was awarded her Ph.D and so that part of the project
was completed.
She is a software developer, working on deconvolution.
Her software relied on an known and measured PSF. Rather inconvenient
due to differing convolution within different tissues.......but just
good enough to get the Ph.D.

I did not complete the project (collecting data from all objectives)
just the 63x 1.2NA objective with 488nm laser.  Because the scope is in
a core facility, I could not address some of the issues brought up.
like the asymmetry above and below the microsphere.
This was only apparent with the pinhole all the way open.  I have the
images some were...I will dig them up if you are still interested. I
have them in a presentation archived.

James Beals
Research Assoc. II
University of Michigan
Mental Health Research Institute
205 Zina Pitcher Place
Ann Arbor, Mi 48109-0720
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On Wednesday, August 18, 2004, at 12:53  PM, Carl Boswell wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi James,
> I'm trying to define the PSF's for our Nikon confocal and came across
> this
> communication of yours from a year ago.  Do you  still have the image
> data
> available online?  The link below doesn't work for me.  Did you finish
> the
> project?
> thanks,
> Carl
>
>
> Carl Boswell, Ph.D.
> Dept. of Molecular and Cellular Biology
> Univ. of Arizona
> 520-626-8469
> 520-621-3709 FAX