CONFOCALMICROSCOPY Archives

July 1997

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Subject:
Re: Fixed and permeabilized cells with fluorescent probes
From:
Beat Ludin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 1 Jul 1997 09:39:08 -0000
Content-Type:
text/plain
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text/plain (15 lines) 
Fixation with pFA is not very reliable for fixing soluble cytoplasmic
proteins (a major proportion of which can still be washed out after
fixation) and probably dextrans also. Adding a detergent will probably
make it even worse. Adding a crosslinking fixative, such as
glutaraldehyde or EGS should make the fixation much more reliable. And,
as Don has already pointed out, make sure that you use a FIXABLE dextran.

Beat


-----------------------------------------------------
Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976
Internet:[log in to unmask] / Compuserve:100102,1527

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