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Sorry, page 3.

On Thu, May 27, 2021 at 9:05 PM Douglas Richardson <[log in to unmask]>
wrote:

> Unfortunately not. It's a single fixed mirror that blocks all 4 laser
> lines.
>
> Component #4 in diagram on Page 4 here:
> https://bcf.technion.ac.il/wp-content/uploads/2017/12/Instruction-LSM700-2017-12-07.pdf
>
>
> -Doug
>
> On Thu, May 27, 2021 at 8:57 PM Model, Michael <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> I never had LSM700 but I wonder if it might be possible to trick it by
>> scanning with two lasers simultaneously... At least one of them must get
>> past the filter. (Apologies for talking without knowing)
>>
>> Mike
>>
>> -----Original Message-----
>> From: Confocal Microscopy List <[log in to unmask]> On
>> Behalf Of Douglas Richardson
>> Sent: Thursday, May 27, 2021 8:42 PM
>> To: [log in to unmask]
>> Subject: EXT: Re: confocal reflectance microscopy with Zeiss LSM700
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>> Post images on
>> https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=04%7C01%7Cmmodel%40KENT.EDU%7C49473550fec1401e87bb08d921718851%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637577594030733559%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&amp;sdata=uUREpBI7nba%2BOC7Cs45RWROXbYaMDjCea9KBWZfm4zM%3D&amp;reserved=0
>> and include the link in your posting.
>> *****
>>
>> The LSM 700 has a fixed laser blocking filter that reflects/blocks all
>> laser light from reaching the detectors. With laser power and gains maxed
>> out (and an empty filter position in front of the detector) you may be able
>> to see some reflection, but you won't get the quality you're used to with
>> the 15/85 beamsplitter. The LSM 710 and 780 had a 80/20 beam splitter for
>> reflection imaging.
>>
>> We recently purchased an LSM 900 that has 100% reflectance for the 488,
>> 561 and 638 lines, but only reflects 20% of 405 light. This lets you do
>> reflectance imaging with the 405 laser only. Maybe upgradable?
>>
>> -Doug
>>
>> On Thu, May 27, 2021 at 8:26 PM Craig Brideau <[log in to unmask]>
>> wrote:
>>
>> > *****
>> > To join, leave or search the confocal microscopy listserv, go to:
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>> > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=04%7C01%7Cm
>> > model%40KENT.EDU%7C49473550fec1401e87bb08d921718851%7Ce5a06f4a1ec44d01
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>> > 000&amp;sdata=3SpQo%2FLufyqh2WU3u7haFgLtgeT4kY5MXh6Px1XmfY4%3D&amp;res
>> > erved=0 Post images on
>> >
>> https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=04%7C01%7Cmmodel%40KENT.EDU%7C49473550fec1401e87bb08d921718851%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637577594030733559%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&amp;sdata=uUREpBI7nba%2BOC7Cs45RWROXbYaMDjCea9KBWZfm4zM%3D&amp;reserved=0
>> and include the link in your posting.
>> > *****
>> >
>> > If the lasers are turning on (confirm this by looking at your sample
>> > and see if you observe cyan or red laser scatter) then there is
>> > probably a filter blocking the laser light from reaching the
>> > detectors. In fluorescence mode a confocal is very particular about
>> > ensuring that laser scatter does not interfere with fluorescent
>> > signal, so to perform reflectance confocal you have to override all
>> these safeties!
>> >
>> > Craig
>> >
>> > On Thu, May 27, 2021 at 5:37 PM Zervantonakis, Ioannis <[log in to unmask]>
>> > wrote:
>> >
>> > > *****
>> > > To join, leave or search the confocal microscopy listserv, go to:
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>> > > ts.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=04%7C01
>> > > %7Cmmodel%40KENT.EDU%7C49473550fec1401e87bb08d921718851%7Ce5a06f4a1e
>> > > c44d018f73e7dd15f26134%7C1%7C0%7C637577594030733559%7CUnknown%7CTWFp
>> > > bGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6
>> > > Mn0%3D%7C3000&amp;sdata=3SpQo%2FLufyqh2WU3u7haFgLtgeT4kY5MXh6Px1XmfY
>> > > 4%3D&amp;reserved=0 Post images on
>> > > https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww
>> > > .imgur.com%2F&amp;data=04%7C01%7Cmmodel%40KENT.EDU%7C49473550fec1401
>> > > e87bb08d921718851%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C63757
>> > > 7594030733559%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2
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>> > > OC7Cs45RWROXbYaMDjCea9KBWZfm4zM%3D&amp;reserved=0 and include the
>> > > link in your
>> > posting.
>> > > *****
>> > >
>> > > Hello,
>> > >
>> > > we are trying to image collagen fibers in 2mg/ml gels (type I rat
>> > > tail)
>> > as
>> > > performed in Figure 2 here:
>> > > https://nam11.safelinks.protection.outlook.com/?url=https%3A%2F%2Fww
>> > > w.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC2717242%2F&amp;data=04%7C01
>> > > %7Cmmodel%40KENT.EDU%7C49473550fec1401e87bb08d921718851%7Ce5a06f4a1e
>> > > c44d018f73e7dd15f26134%7C1%7C0%7C637577594030733559%7CUnknown%7CTWFp
>> > > bGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6
>> > > Mn0%3D%7C3000&amp;sdata=TPhNB6pv3OCwFwLWMxtwGDgDoho8%2FcqOJg%2FV6rXV
>> > > w6o%3D&amp;reserved=0
>> > >
>> > > We have been able to do reflectance on a Leica TCSP8 with a 15/85
>> > > beamsplitter after collecting light reflectance from the 488nm
>> > excitation,
>> > > but don't get any signal in the Zeiss LSM 700 (we have tried both
>> > > the
>> > 640nm
>> > > laser and 488nm).
>> > >
>> > > Thank you,
>> > > Yannis
>> > >
>> >
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>