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Does anyone know how to get ROIs from .lif files?

We are doing FRAP experiments with a Leica SP8.  We are getting good FRAP analysis results with ImageJ by estimating the location of the ROIs, but would like to use the precise ROIs defined when imaging.  It appears these are in the metadata but don't know how to convert the Leica coordinates, which have negative numbers, to ImageJ coordinates.  Also, there is a ROI importer button in BioFormats, but it always throws an exception.

Any info or help would be appreciated.

Thank you.

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
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