Dear Haixin,

    Another idea might be to use radiolabelling techniques.
For example, add tritiated water to your fungi and then
rapidly freeze them and do dry autoradiography.  I don't
know if this would actually work for imaging water, and
the rapid-freezing technique is not for the faint-hearted,
but it did work well for imaging acetylcholine localization,
providing subcellular resolution.
  See: Masland and Mills, 1979.  J. Cell Biology 83:159-178.

Good Luck,
Don

Donald M. O'Malley, Ph.D.
Department of Biology
414 Mugar Hall
Northeastern University
Boston, MA 02115
[log in to unmask]
www.omalleylab.neu.edu


|
|
|  Hi Gurus,
|  We want to know which part/cells of tissue takes water up first in a
|  fungal system. I am  wondering if somebody in this group knows some
|  techniques or some dyes (water tracer dye?) that could help us to do
|  this work?
|  I would very much appreciate any response.
|
|  Haixin Xu  Ph.D.
|  Department of Botany and Plant Pathology
|  Michigan State University
|  East Lansing, MI 48824
|
|