Hi,
Raman is going to become as available as regular confocal. As a matter of
fact, it is on the same price scale but can do both confocal and chemical
imaging. It is an analytical technique which works well alongside
fluorescence, but you need to collect the Raman signal separately. I just
came back from PITTCON (THE major analytical chemical meeting) and some
companies are collecting the signal in the near UV, others in the near IR.
A good starting point is Jack Koenig's book "Spectroscopy of Polymers" (Am.
Chem. Soc.. Washington DC, 1992). I just saw him at PITTCON and he said
that the new edition is either just coming out or about to come out.
The move to Raman is part of the merging of microscopy and spectroscopy.
There are now several interesting true hybrid instruments which permit
chemical as well as microscopy imaging. One is the Continuum from
Spectra-Tech (Shelton, CT); the other is a series built on the DuraScope
from SensIR (Danbury, CT). I had a chance to teach a microscopy class to
the IR specialists from SpectraTech in January, so really had a chance to
have my hands on the system. In addition to running full FT-IR chemical
spectra, I was able to do low power darkfield. They also have regular
phase and DIC objectives available (the Phase was a bit tricky to
implement) and have just introduced a fluorescence module.
I will be doing a review for American Lab (Watch for Am Lab: "Focus on
Microscopy") and will post pertinent excerpts for you within the next few
weeks.
Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.
125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: [log in to unmask]
Visit our web site <http://www.MME-Microscopy.com/education>
At 03:00 AM 3/2/00 +0000, Jose Feijo wrote:
>Since the subject was raised, could anyone point me out a good paper or
internet source to understand Raman microscopy, and how to make it work? On
the side, from the gurus, how much should we expect from it in the future?
Is it like, it's going to substitute something already available, or
otherwise it will complement specific aspects of visualisation of some
special biological structure? What does it involve, special lasers, special
optics, special computers?
>
>Thanks in advance
>Jose
>
>
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