At 08:42 24.08.00 +0930, you wrote:
>We have used gelatine embedding to hold small bits of tissue (autonomic
>ganglia) for sectioning prior to doing immunolabelling for TEM... After
>a lot of mucking around, we ended up using a 20% solution of molten
>gelatine dissolved in PBS to embed the prefixed tissue. The big trick
>then is to refix the gelatine block containing the tissue so that
>everything is held on place and the gelatine is tough enough to
>withstand sectioning on a Vibratome (or whatever you section with). (The
>details are in Murphy et al (1998) J Comp Neurol,398:551-567). I would
>expect that you would have to play around with the length of fixation
>and so on to suit your tissue and the size of your block etc...
>
>Hope that helps
>
>IAN
>
>
>Derek Schulze wrote:
>>
>> I'm trying to use gelatin to embed coral tissue which is 2 cell layers
>> thick in order to view the tissue edge-on with our confocal. What
>> concentration should the gelatin be?  Does anyone have a protocol to
>> suggest? I've tried up to 6% gelatin but the embedded tissue pulls away
>> from the gelatin when sliced.
>>
>> Lena
>
>--
>Professor Ian Gibbins
>Anatomy & Histology
>Flinders University of South Australia
>GPO Box 2100, Adelaide, SA 5001
>Australia
>
>Phone:  +61-8-8204 5271
>FAX:    +61-8-8277 0085
>Email:  [log in to unmask]
>

Dr. Arthur Schuessler
TU Darmstadt
FB10 Botanik
Schnittspahnstr. 10
D-64287 Darmstadt
Germany

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