Dear Beat,

we have been unsuccesfull fusing DSred to PH domains that worked nice
with CFP, GFP and YFP.
However, I just talked to Roger Tsien who probably has solved the problem
(manuscript submitted) but I of course cannot tell you. I suggest you
contact him directly to get the scoop.

regards, Kees


Beat Ludin wrote:

> Hello everybody
>
> Have you worked with DsRed/RFP fusion constructs, successfully or
> unsuccessfully? If so, it would be great if you could participate in
> a little survey.
> I have been approached by several researchers having trouble to get
> fluorescent fusion constructs with DsRed (in most cases, the same
> construct was fine when a different fluorescent protein was used). I
> also know of others who were successful, but so far no pattern has
> emerged from the small data set I have available. To get more data, I
> would like you to answer as many of the questions below as possible
> (of course, I'm not asking you to disclose any information you wish
> to keep to yourself for the time being). To avoid cluttering the
> newsgroup, please reply to me privately ([log in to unmask]). I will post
> a summary to the newsgroup at the end of the survey.
>
> Thanks in advance,
>
>         Beat
>
> -------------------
>
> What protein was DsRed fused to?
>         Type (soluble, transmembrane, ...)?
>         Subcellular location?
>
> What did the fusion construct look like?
>         (N)DsRed-XXXX(C)
>         (N)XXXX-DsRed(C)
>         (N)XX-DsRed-XX(C)
>
> Was a linker included?
>         Type or sequence?
>
> What systems was the fusion construct expressed in?
>         Temperature?
>
> Was the fusion protein fluorescent?
>
> Did the fusion partner appear functional?
>
> Imaging setup used?
>
> -------------------
>
> LIFE IMAGING SERVICES
>                - visit our web site at http://www.lis.ch
> +-----------------------------------------------------------
> | Dr. B. Ludin
> | Life Imaging Services     fon ++41 (0)79 235 7154
> | Muehletalweg 22           fax ++41 (0)860 622 963 160
> | CH-4600 Olten             [log in to unmask]
> | Switzerland               http://www.lis.ch

--

Kees Jalink Ph.D.
The Netherlands Cancer Institute, dept. of Cell Biology H1
Plesmanlaan 121, 1066CX Amsterdam, the Netherlands
020-5121933 (tel office)/ ...1947 (tel lab) / ...1944 (fax) / [log in to unmask]
(email)
at home: Paulus Potterlaan 5, 2102CC Heemstede
0235-476047 / [log in to unmask]