Sender: |
|
Date: |
Thu, 31 Aug 2000 15:15:37 -0300 |
Reply-To: |
|
Subject: |
|
MIME-Version: |
1.0 |
Content-Transfer-Encoding: |
8bit |
Content-Type: |
text/plain; charset=iso-8859-1 |
Organization: |
Dept Farmacologia / Universidade Federal de Minas Gerais |
From: |
|
Parts/Attachments: |
|
|
Maybe it would be easier (better) to change the green to a brighter
color (some sort of "fluorecent" green) that stands out better against a
dark background.
--Chris
Ken Takeda wrote:
>
> Dear Confocalists,
>
> This is probably a trivial question, but...
>
> We would like to modify the (black) background in .pic files
> generated using Lasersharp TimeCourse software on a MRC 1024 system
> showing cells expressing membrane-localized GFP-tagged proteins - any
> tips on how to do this ?
> We would like to end up with the (same) green GFP pixels on a
> non-black background, like white or grey, in an effort to have
> enhanced visibility of the GFP.
>
> Thanks in advance for your help. Ken
>
> Ken Takeda
> ____________________________________________________________________________
> UMR CNRS 7034
> Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires
> Université Louis Pasteur de Strasbourg, Faculté de Pharmacie
> B.P. 24, 74 route du Rhin, F-67401 Illkirch, France
>
> Phone Office: [+33] (0)3 88 67 68 94
> Phone Secretariat: [+33] (0)3 88 67 69 30
> Phone Laboratory: [+33] (0)3 88 67 69 29
> FAX: [+33] (0)3 88 66 46 33
> E-mail: [log in to unmask]
> http://www-umr7034.u-strasbg.fr
--
Christopher Kushmerick <[log in to unmask]>
Dept. Farmacologia tel 55 31 499 2707
Inst. Ciencias Biologicas fax 55 31 499 2695
Universidade Federal Minas Gerais
Av. Antonio Carlos 6627, Pampulha
31270-901 Belo Horizonte, MG
Brasil
|
|
|