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August 2000

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: questions on Cy3/permeabilisation
From:
Martyn Evans <[log in to unmask]>
Date:
Mon, 14 Aug 2000 10:12:32 +0100
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Confocal Microscopy List <[log in to unmask]>
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Hi All - Ian's last reply was very interesting, as I've been wondering about
detergent alternatives to permeabilisation for antibodies some time.  Any
opinions among people about comparative merits of Triton, Tween, saponin, on
morphology if you have to go the detergent route.  Also what alternatives are
there - freeze-thaw? for thick specimens (say 50 -80 microns vibratome cut brain
sections).  Ian, could you give a reference or detailed protocol for the DMSO
method?  How uniform is the labelling through the thickness of the section?
regards,

Martyn Evans
SmithKline Beecham
Harlow UK

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