Hello everyone;
   I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy.  I can clearly see the fungus using two filter sets: a UV set (ex/em 365/>420) and a blue filter set (ex/em 470/>515).  While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light.  is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics?  I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.

thanks in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada   K1A 0C6
Phone:  (613) 759-1760
Fax:  (613) 759-1701
E-mail:  [log in to unmask]