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Tue, 22 Aug 2000 08:10:54 +0930 |
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Flinders University |
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Hello Martyn
Did you get my extended meesage to the confocal list about protocols? If
not, or if you need more info, let me know...
IAN
Martyn Evans wrote:
>
> Hi All - Ian's last reply was very interesting, as I've been wondering about
> detergent alternatives to permeabilisation for antibodies some time. Any
> opinions among people about comparative merits of Triton, Tween, saponin, on
> morphology if you have to go the detergent route. Also what alternatives are
> there - freeze-thaw? for thick specimens (say 50 -80 microns vibratome cut brain
> sections). Ian, could you give a reference or detailed protocol for the DMSO
> method? How uniform is the labelling through the thickness of the section?
> regards,
>
> Martyn Evans
> SmithKline Beecham
> Harlow UK
--
Professor Ian Gibbins
Anatomy & Histology
Flinders University of South Australia
GPO Box 2100, Adelaide, SA 5001
Australia
Phone: +61-8-8204 5271
FAX: +61-8-8277 0085
Email: [log in to unmask]
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