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August 2000

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Confocal Microscopy List <[log in to unmask]>
Subject:
Coral tissue embedding in gelatin
From:
Derek Schulze <[log in to unmask]>
Date:
Wed, 23 Aug 2000 14:18:15 -0400
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Confocal Microscopy List <[log in to unmask]>
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I'm trying to use gelatin to embed coral tissue which is 2 cell layers
thick in order to view the tissue edge-on with our confocal. What
concentration should the gelatin be?  Does anyone have a protocol to
suggest? I've tried up to 6% gelatin but the embedded tissue pulls away
from the gelatin when sliced.

Lena

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