Dear Ray,

You could use a Sykes-Moore chamber. this is a very simple chamber available
from Bellco glass Co.

"HTTP://WWW.BELLCOGLASS.COM/sect2.htm"

(No commercial affiliation with Bellco Glass)

Stephen H. Cody,
Colon Molecular and Cell Biology Laboratory,
Ludwig Institute for Cancer Research,
Post Office Royal Melbourne Hospital,
Parkville, Victoria 3050, Australia.

Tel: 61 3 9341 3155   Fax: 61 3 9341 3104
email: [log in to unmask]
www.ludwig.edu.au/confocal

> ----------
> From:         ray hester[SMTP:[log in to unmask]]
> Reply To:     Confocal Microscopy List
> Sent:         Thursday, August 24, 2000 12:29 AM
> To:   [log in to unmask]
> Subject:      Upright scope with closed culture system and 100x objective?
>
> Hi,
>
> Is anyone using an upright fluorescence microscope to analyze viable cells
> in a closed culture system with a 100x oil objective. i.e., the cells will
> have to be upside down on the upper cover glass and the chamber filled
> with
> culture fluid?
>
> If so, did you have to make your culture holder or are there commercially
> available systems that will accomplish this?  The Bioptechs catalog shows
> the FCS3 which would permit this but I'm not sure if it's in production
> yet.
>
> Thanks for any help.
>
> Ray
>
> Raymond B. Hester, Ph.D.
> Research Cytometry Laboratory
> CSAB 357, Biotechnical Services
> University of South Alabama
> Mobile, AL 36688
> Voice:    (334) 460-6029
> FAX:      (334) 460-6073
> E-mail:   [log in to unmask]
>