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Date: | Mon, 28 Aug 2000 14:11:16 -0400 |
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We are contemplating a Zeiss LSM510 and are trying to think through the
laser configuration. They can only demo Option 1.
Option 1: Argon, Green HeNe, Red HeNe (457, 488, 512, 543, 633)
Option 2: Argon/Krypton, Red HeNe (or Red Diode) (488, 568, 633)
Option 3: Argon, Nd:YAG, Red HeNe (or Red Diode) (488, 532, 633)
Is a 1mW Green HeNe of any use in "typical" sample scenarios ie; not bright
actin or tonnes of GFP? Is the 568 line of an Argon Krypton more useful
(power vs wavelength)? What about a diode pumped Nd:YAG 20mW @ 532nm?
Any comments welcome.
- Derek Schulze
Flow Cytometry and Confocal Microscopy Core Facility
Cancer Research Labs
353 Botterell Hall
Queen's University
Kingston, Ontario
K7L 3N6
Canada
http://meds.queensu.ca/medicine/crl/
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