CONFOCALMICROSCOPY Archives

August 2000

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Subject:
From:
Derek Schulze <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 28 Aug 2000 14:11:16 -0400
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We are contemplating a Zeiss LSM510 and are trying to think through the
laser configuration.  They can only demo Option 1.

Option 1: Argon, Green HeNe, Red HeNe (457, 488, 512, 543, 633)
Option 2: Argon/Krypton, Red HeNe (or Red Diode) (488, 568, 633)
Option 3: Argon, Nd:YAG, Red HeNe (or Red Diode) (488, 532, 633)

Is a 1mW Green HeNe of any use in "typical" sample scenarios ie; not bright
actin or tonnes of GFP?  Is the 568 line of an Argon Krypton more useful
(power vs wavelength)?  What about a diode pumped Nd:YAG 20mW @ 532nm?

Any comments welcome.

- Derek Schulze

Flow Cytometry and Confocal Microscopy Core Facility
Cancer Research Labs
353 Botterell Hall
Queen's University
Kingston, Ontario
K7L 3N6
Canada

http://meds.queensu.ca/medicine/crl/

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