After a few messages pro-HeNe, I will be on the other side...

I NEVER use the HeNe laser for confocal fluorescence imaging. I am running a
Zeiss LSM410 with 543 HeNe laser + 488/568/647 ArKr laser. Why would you use
a weak "peak mismatched" 543 laser to excite green or red fluos when you
have powerful 488 and 568 lines? Actually i use the HeNe laser only to get a
transmission image to be able to superimpose morphology to fluorescence
because the contrast image is slightly better with the 543 line.


***********************
Hugo Dilhuydy, ing.
Laboratoire de microscopie
Centre de recherche
Institut universitaire de gériatrie de Montréal
4565 chemin Queen-Mary
Montréal (Québec)
H3W 1W5 CANADA
Tél: (514)-340-3540 poste 3222
Fax: (514)-340-2801
http://www.criugm.qc.ca

----- Original Message -----
From: Derek Schulze <[log in to unmask]>
Newsgroups: bit.listserv.confocal
To: <[log in to unmask]>
Sent: Monday, August 28, 2000 2:11 PM
Subject: Green HeNe laser power


> We are contemplating a Zeiss LSM510 and are trying to think through the
> laser configuration.  They can only demo Option 1.
>
> Option 1: Argon, Green HeNe, Red HeNe (457, 488, 512, 543, 633)
> Option 2: Argon/Krypton, Red HeNe (or Red Diode) (488, 568, 633)
> Option 3: Argon, Nd:YAG, Red HeNe (or Red Diode) (488, 532, 633)
>
> Is a 1mW Green HeNe of any use in "typical" sample scenarios ie; not
bright
> actin or tonnes of GFP?  Is the 568 line of an Argon Krypton more useful
> (power vs wavelength)?  What about a diode pumped Nd:YAG 20mW @ 532nm?
>
> Any comments welcome.
>
> - Derek Schulze
>
> Flow Cytometry and Confocal Microscopy Core Facility
> Cancer Research Labs
> 353 Botterell Hall
> Queen's University
> Kingston, Ontario
> K7L 3N6
> Canada
>
> http://meds.queensu.ca/medicine/crl/