CONFOCALMICROSCOPY Archives

August 2000

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Subject:
From:
Chris Kushmerick <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 31 Aug 2000 15:15:37 -0300
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Maybe it would be easier (better) to change the green to a brighter
color (some sort of "fluorecent" green) that stands out better against a
dark background.

--Chris


Ken Takeda wrote:
>
> Dear Confocalists,
>
> This is probably a trivial question, but...
>
> We would like to modify the (black) background in .pic files
> generated using Lasersharp TimeCourse software on a MRC 1024 system
> showing cells expressing membrane-localized GFP-tagged proteins - any
> tips on how to do this ?
> We would like to end up with the (same) green GFP pixels on a
> non-black background, like white or grey, in an effort to have
> enhanced visibility of the GFP.
>
> Thanks in advance for your help.  Ken
>
> Ken Takeda
> ____________________________________________________________________________
> UMR CNRS 7034
> Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires
> Université Louis Pasteur de Strasbourg, Faculté de Pharmacie
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>
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--
Christopher Kushmerick  <[log in to unmask]>

Dept. Farmacologia                              tel 55 31 499 2707
Inst. Ciencias Biologicas                       fax 55 31 499 2695
Universidade Federal Minas Gerais
Av. Antonio Carlos 6627, Pampulha
31270-901 Belo Horizonte, MG
Brasil

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