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Date: | Fri, 11 Aug 2000 12:26:57 -0500 |
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The other big problem with -20 freezers is that they so often are
"frost-free" these days. The auto defrost cycles on these types of
freezers are very tough on antibodies and sensitive enzymes. I
believe Jackson recommends diluting their antibodies in 50% glycerol
if you want to store at -20 so that they don't "freeze" but yet are
kept cold. I don't know if that limits the damage by a frost-free
freezer but do know that the molecular biology types all stress that
their enzymes diluted in glycerol are still very labile if stored in
a frost-free freezer.
Tom
>Tom Phillips wrote:
>>
>> I was surprised by Martin's experience. I buy lots of Jackson
>> antibodies (FITC, Cy3, Cy5, HRP; goat and donkey anti-mouse and
>> anti-rabbit) and generally aliquot them out as 10 ul/tube and freeze
>> and store at -80. Once thawed, I dilute and use that day and throw
>> any extra away. They have been stable for years this way. Tom
>
>The differences in our experiences might have to do with our respective
>speeds of freezing: -80 vs -20 (which was what I tried); 10 ul vs ~100
>ul (which I used). Faster freezing is better, in my
>experience--probably allows less time for the liquid to partition into
>salt-rich and salt-poor fractions.
>--
>Martin Wessendorf, Ph.D. office: (612) 626 0145
>Associate Professor lab: (612) 624 2991
>Dept. Neuroscience Preferred FAX: (612) 624 8118
>University of Minnesota Dept FAX: (612) 626 5009
>Minneapolis, MN 55455 e-mail: [log in to unmask]
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)
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