Hi Kathryn,

Have you tried Citiflour?  Ted Pella, Inc has it. You use it in place of
mounting media.

Diane G. Miller
[log in to unmask]
503-627-0130

Kathryn Spencer <[log in to unmask]> wrote in message
news:3.0.6.32.20000727131105.008413b0@scripps.edu...
> Hello All!
>          We are trying to look at GFP-tagged proteins in transfected cells
by 4D
> confocal. Even though we reduce the laser power, open the iris, reduce
> number of sections, decrease the gamma, etc., the GFP signal fades quickly
> (it's not terribly strong to begin with, but is enough to see easily). The
> cells seem healthy and happy enough. Any suggestions how we can keep the
> GFP fluorescence from fading?
>         Kathy Spencer
>
>
>
>
>
> Kathy Spencer
> The Scripps Research Institute
> 10550 N. Torrey Pines Road
> IMM 24
> La Jolla, CA 92037
> (858) 784-9372
> [log in to unmask]
>