Forgive me for cluttering the list, I don't have the original poster's address

Mario's reply reminded me; 15 microns IS quite large; there is a concern that particles greater than 5-7 microns may get trapped in capillary beds, particularly those of the lungs.  So they may not be recirculating & getting through to your tissue.  Or is that the reason FOR using such large spheres?  (I've always wondered how blood flow analysis is done with microspheres.)

Richard

> [log in to unmask] 8/2/00 9:30:25 AM >>>
Andras,

>  >
>>>Hi all,
>>>
>>>
>>>We are looking at performing a quantitative blood flow analysis by
>counting fluorescent microspheres (15 microns size, made of polystyrene and
>coated with flourescent dye) in the nerve of a rat after injection into the
>abdominal aorta.

These are very large spheres and carry a lot of dye.  . . .

Richard Thrift

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