We have recently been imaging 15um fluorescent beads and there seems to be
an "artifact" in the images.  When a 3-D image of a bead is collected and
then viewed in an xz or yz view there is a fluorescent "shadow" on the
side of the bead away from the objective.  It's very bright and just
beyond where the edge of the bead should be.  Has anyone else experienced
this?  I'm aware of the aberrant stretching of the image in the z
dimension, but this seems to be somewhat different as we do not get the
same results with other shapes. Could this be due to the spherical nature
of the beads?  I have images, if anyone is interested.

Joe Shope

Department of Biology
Utah State University
Logan, Utah
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(435) 797-0866