I have a question concerning deconvolution. I am wondering if it is
possible and reasonable to deconvolve confocal (or partially confocal)
images obtained in backscattered light mode. I assume that in such a
case PSF function would be dependent on size of backscattering
particles present in the sample and pace of their Brownian movement.
Am I correct? If so, how can I determine a model PSF which might be
used later for a deconvolution? Surely I cannot obtain it
experimentaly relying on an image of a single reflective bead since
BSL impulse response function depends strongly on the neighborhood of
the bead observed. What about blind deconvolution?
Any ideas?
Bartek Rajwa
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