Not strictly confocal:
We have tried to track the distribution of a FITC-labelled Ab after
injection into the cerebellum of P7 rats. We were very dissapointed to find
virtually NO fluorescence at 2 days after injection of 1 ul of a standard
secondary Ab stock solution. The injection was under visual control and we
were able to identify the needle track on the sections. The animals were
perfused by ringer followed by formaldehyde (all buffered at pH7.2) and the
brains cryosectioned. Could it be that the FITC fluorochrome has been
degraded so quickly in the animal? Next we will look 2 hrs. after injection
but we would also like to know about 2 days. Any comments, suggestions welcome.

Christian
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Christian Broesamle, PhD
Brain Research Institute
Department of Neuromorphology (Gruppe Schwab)
Federal Institute of Technology and
University of Zurich
Switzerland
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Tel: +41 1 635 3216
Fax: +41 1 635 3303
Street address:
Winterthurer Strasse 190
CH-8057 Zurich
Switzerland
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