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January 2001

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Nuclear fluorochrome
From:
Cristiano Rumio <[log in to unmask]>
Date:
Fri, 19 Jan 2001 16:58:15 +0100
In-Reply-To:
<[log in to unmask] c.ac.uk>
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Confocal Microscopy List <[log in to unmask]>
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At 15.40 19/01/01 +0000, you wrote:
>Hi all,
>
>Does anyone know of a nuclear "counterstain" suitable for use with an argon
>laser which gives a green fluorescence (or any colour other than red).
>Propidium iodide is thus out, and DAPI, I believe, won't work.
>
>Many thanks
>
>Tony

If you have the 633 or 647 line you can use TOTO3. It works great!

Cristiano


Dr. Cristiano Rumio
Department of Human Anatomy
Via Mangiagalli 31
20133 Milan
Italy
Tel. 0039-0270646243
Fax. 0039-022364082
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