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Mon, 22 Jan 2001 12:00:58 +0000 |
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Hi,
We tried a bunch of the syto dyes and number 13 worked well for us.
Craig.
At 17:44 19/01/01 +0100, you wrote:
>Hi Tony,
>
>Try Sytox Green for fixed cells (exc 488 , green emission). For live
>cells have a look of all Syto- carbocyanines. All of them are from
>Molecular Probes. They work well if you use at low concentrations.
>
>
>Susanna
>
>"tony whyte (BI)" wrote:
> >
> > Hi all,
> >
> > Does anyone know of a nuclear "counterstain" suitable for use with an argon
> > laser which gives a green fluorescence (or any colour other than red).
> > Propidium iodide is thus out, and DAPI, I believe, won't work.
> >
> > Many thanks
> >
> > Tony
_______________________________________________
Dr. Craig J Daly,
Research Fellow,
Wolfson Building (room 448),
West Medical Building (Lab 440),
University of Glasgow,
Glasgow G12 8QQ
Scotland.
Tel. 0141 330 3920; Fax. 0141 330 2923
www.neuroscience.co.uk/staff/cdaly.htm
www.cardiovascular.org
www.adrenoceptor.com
www.vascan.net
________________________________________________
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