CONFOCALMICROSCOPY Archives

March 2001

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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: immunofluorescence MetOH GFP
From:
Rosemary White <[log in to unmask]>
Date:
Thu, 8 Mar 2001 08:46:05 +1100
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Confocal Microscopy List <[log in to unmask]>
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Dear all,

Taking this thread a little further, does anyone have a good protocol for
resin-embedding GFP tagged tissue?  Ethanol or acetone dehydration kills
the GFP fluorescence at around 95% level, even after paraformaldehyde
fixation, so we were thinking of using anti-GFP to localise it.  But if
there is a method for preserving GFP fluorescence for thick (1-2 micron)
sectioning, I would love to hear about it.

Thanks much,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone  61-2-6246 5475
fax       61-2-6246 5000
email   [log in to unmask]

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