Hello, group --

I'd like to extend the colocalization questions from last week once
more, even at the risk of moving a bit away from the confocal theme
of the listserv.  I read over most of the suggested texts from that
discussion, but didn't come across an answer to this:

We are trying to determine if a blue DAPI-range dye and a red dye are
going to the same location.  We're lucky to have good separation of
ex/emit peaks, but don't have access to a confocal that can excite
the blue dye.  However we do have access to another microscope with
deconvolution software and a source that can access the uv/blue
range.  Are there additional issues to consider for determining
colocalization with a deconvolution microscope?  Will this change the
analysis at all?

Thanks in advance --

Dan Harrington
_____________________________________________________________
Daniel Harrington
E-mail: [log in to unmask]
Northwestern University
Department of Materials Science and Engineering
2225 N. Campus Dr
Evanston, IL 60208-3108

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