CONFOCALMICROSCOPY Archives

March 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
David Knecht <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 5 Mar 2001 15:22:12 -0500
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>Dear Barbara- I have been looking into this and Ludl has a much
>cheaper stabilized source.  Any negative thoughts? Also, is there
>any real downside to buying a stabilizer as opposed to going with a
>Xenon bulb?  My reading of the discussion is that you don't need
>anything fancy with Xenon in terms of power supply.  Dave



>Steve,
>
>OptiQuip has new stabilized Hg light sources.  Many years ago I was the
>East Coast microspectrophotometry specialist for Zeiss and used stabilized
>HBO's but they have been very expensive.  OptiQuip has extensive experience
>in this area and makes lamp housings to fit most microscopes.  If
>interested, contact: Mel Decker - [log in to unmask]
>
>Caveat: no vested interest.
>
>Best regards,
>Barbara Foster
>Microscopy/Microscopy Education
>125 Paridon Street, Suite 102
>Springfield, MA 01118
>PH: 413-746-6931  FX: 413-746-9311  Web: www.MME-Microscopy.com/education
>
>"Why didn't they teach us that sooner?"  ... probably because no one
>thought to call MME about customized, on-site courses.  Offered in all
>areas of microscopy, sample prep,and image analysis, they make an immediate
>impact on your productivity.
>@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
>
>
>
>
>At 09:41 AM 2/28/01 +1100, Stephen Cody wrote:
>>  >From my observations of using both types of light source, the Xenon seems
>>far more stable with time. You don't notice the fluctuations that you get
>>with Hg bulbs. This is important for quantification, such as dual excitation
>>ratio methods (eg. Fura-2).
>>
>>Stephen H. Cody,
>>Colon Molecular and Cell Biology Laboratory,
>>Ludwig Institute for Cancer Research,
>>Post Office Royal Melbourne Hospital,
>>Parkville, Victoria 3050, Australia.
>>
>>Tel: 61 3 9341 3155   Fax: 61 3 9341 3104
>>email: [log in to unmask]
>>www.ludwig.edu.au/confocal
>>
>>
>>>  ----------
>>>  From:         Tom Phillips[SMTP:[log in to unmask]]
>>>  Reply To:     Confocal Microscopy List
>>>  Sent:         Wednesday, 28 February 2001 2:08
>>>  To:   [log in to unmask]
>>>  Subject:      Re: Xenon lamps
>>>
>>>  All true but I believe one disadvantage is the peak illumination is
>>>  less for some fluorochromes such as DAPI.  We see a difference in our
>>  > two systems that have Hg or Xe.
>>  >
>>  >

--

************************************************************
Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd.   U-125
Storrs, CT 06269-3125
[log in to unmask]
860-486-2200      860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************

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