CONFOCALMICROSCOPY Archives

March 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
best serum free media for lymphocytes
From:
Heather Medbury <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Mar 2001 14:36:48 +1100
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Hi FLOWers,

In your opinion what is the best serum free media to use for culturing lymphocytes in. I will be doing intracellular cytokine staining of antigen stimulated lymphocytes but want to avoid using serum.

Any comments on intracellular cytokine staining methods is also welcome.

Heather

Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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     All experiments are preliminary

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  "In his heart a man plans his course
    but the Lord determines his steps"

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