What about phalloidin bound to FITC or Cy5 for f-actin?  We use this a lot.
 The incubation time is no more than an hour and can be combined with the
primary antibody incubation.
http://www.aecom.yu.edu/aif/instructions/immunofluor/protocol_bailly.htm

At 06:16 AM 7/19/2001 -0400, you wrote:
>To fit in with the other fluors, it should emit in the FITC region
>or in the far red region.  Ideally it would be a nuclear stain (but we
>don't have a uv source), but not essentially.  I contacted Molecular Probes
>and they recommended some membrane probes (eg FM 4-64, RH 414), but I
>thought I would try for something a little cheaper if possible.  Has anyone
>any recommendations/experience?

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
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