Dear Listmembers,

I am currently using a Zeiss LSM510 with an inverted Axiovert 100 M, as well
as an CCD imaging system build around an Axiovert S100 TV.
Both systems I use to measure physiological ion concentrations over longer
periods of time (10-30min), but I have noticed that with both microscopes
the focus isn't stable and is completely lost over the course of an
experiment. With the confocal it's about 5mum in 10min, going downwards.
Since the cells are only max 5mum thick, this is intolerable. Initially I
thought that on the imaging system the focus instability might be due to a
weak table that responds to weight differences during perfusion. But even
without perfusion, as well as on the confocal with a much more stable table
the focus drifts. I am still waiting for a detailed response from Zeiss, but
the first answer was that this seems to be an hitherto unknown problem. So
my question is:

Has anybody experienced similar problems with focus stability on an Axiovert
100?

Or is it bad coincidence that both my microscopes happen to be faulty and I
just have to buy two "greatly improved Axiovert 200 with maximum
stability"...

Cheers

Frank


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