>
> I am currently using a Zeiss LSM510 with an inverted Axiovert 100 M, as well
> as an CCD imaging system build around an Axiovert S100 TV.
> Both systems I use to measure physiological ion concentrations over longer
> periods of time (10-30min), but I have noticed that with both microscopes
> the focus isn't stable and is completely lost over the course of an
> experiment. With the confocal it's about 5mum in 10min, going downwards.
>
> Has anybody experienced similar problems with focus stability on an Axiovert
> 100?
>

Do you mean that the turret descends? If so, then I can
report a similar problem that we had with an Axiovert 135, which is similar
to the axiovert 100. One other question -- if you lower the objectives, do
they "crash" downward at a certain point?

If this is the case then I can describe what I did to fix it -- it may  of
interest to any listmembers who have a axiovert based confocal.

--aryeh
Aryeh Weiss                          | email: [log in to unmask]
Department of Electronics            | URL:   http://optics.jct.ac.il/~aryeh
Jerusalem College of Technology      | phone: 972-2-6751146
POB 16031                            | FAX:   972-2-6751275
Jerusalem, Israel                    | ham radio: 4X1PB/KA1PB