Dear Florian,

I have used low melting point Agarose to mount zebrafish, with a water
immersion lens and found it to be an excellent mountant. I always mount the
fish close to the coverslip anyway, so that the agarose will have a minimal
effect. I've used 1% up to 4% and noticed no difference in image quality
with the fish next to the coverslip.

Stephen H. Cody,
Colon Molecular and Cell Biology Laboratory,
Ludwig Institute for Cancer Research,
Post Office Royal Melbourne Hospital,
Parkville, Victoria 3050, Australia.

Tel: +61 3 9341 3155 (BH)   +61 3 9341 3158 (@work AH)
Fax: +61 3 9341 3104
email: [log in to unmask]
www.ludwig.edu.au/colonmolecular/cody.html


> ----------
> From:         Florian Ulrich[SMTP:[log in to unmask]]
> Reply To:     Confocal Microscopy List
> Sent:         Monday, 10 September 2001 13:32
> To:   [log in to unmask]
> Subject:      Refractive Index and Mounting Medium
>
> --
> Dear all,
>
> does anyone of you know the refractive index of 1% low melting
> agarose in water? My second question is about mounting medium: I want
> to do antibody staining on zebrafish embryos; I use a FITC-Alexa
> secondary antibody. I used 1% low-melting agarose, but maybe this s
> not the optimal medium... so, if anybody has experience with this, I
> would be happy about suggestions.
>
> Thank you very much,
>
> Florian Ulrich
> Heisenberg Group
> MPI-CBG, Dresden
> Pfotenhauerstrasse 108
> 01099 Dresden
> Germany
> 0049 - 351 - 2102689
>