Jessica Wagner wrote:

> Is it realistic to try to colocalize a 0.5
> um object with fluorophores as widely spaced as the Alexa488 & Cy5?

Yes.  We've published images of triple-labeled (Bodipy, rhodamine, and
Cy5) nerve axons in that size range, and not seen discernable shift in
the x-y plane.  However, that was done using tissue mounted in DPX and
an 1.4 na oil-immersion lens.

Good luck!

Martin Wessendorf

--
Martin Wessendorf, Ph.D.                       office:  (612) 626 0145
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