Is it an inverted microscope? If it is, then you are seeing the cells from
top to bottom as you focus through them by raising the stage. On a regular
microscope it's the other way round.

Lesley Weston.


> From: Frank Lee <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Date: Mon, 3 Dec 2001 12:58:23 -0500
> To: [log in to unmask]
> Subject: Raising and Lowering stage
>
> Hi,
>
> I have a basic question that needs to be sorted out. I have a coverslip
> with cells growing on it. When I mount it on a glass slide, I need to
> turn it upside down. Next I put it on the stage of an upright
> microscope. The question is, when I raise the stage, am I seeing the
> cells from top to bottom or bottom to top? I think it's bottom to top
> because the cells are now upside down (coverslip is turned upside down
> when mounting). Am I correct? Thanks for the help.
>
> Frank Lee