Dear Readers,
in the Glossary of Barbara Foster's excellent book "Optimizing Light
Microscopy for  Biological and Clinical Labs," she distinguishes
between depth of field and depth of focus.  What is depth of focus
dependent on, besides the NA of the objective?  How can one measure
it?   I was asked to provide the depth of focus for an
epi-fluorescence image taken on one of our microscopes.  Can I do
this?

Thank you for your help.

Carol
--
Carol Bayles
Microscopy,  Imaging & Fluorimetry (MIF)         Confocal and
Multiphoton Microscopy
Bioresource Center                       Nanobiotechnology Center
160 Biotech Bldg                                141 Clark Hall
http://www.brc.cornell.edu              http://www.nbtc.cornell.edu

Cornell University     Ithaca NY 14853
607-254-4860      607-254-4847  fax
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