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One trick that is often used with zebrafish embryos is to pipette a warm
solution of agarose gel onto them, then let the gel set up.  It's not
particularly elegant, but the gel will hold the fish/tissue in place.
Of course this solution won't work if you're trying to do nasty things
to it from above, but otherwise works quite well.
Barbara Foster wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Listers,
>
> A question from a colleague in Ireland.  Please reply directly to Sheila at:
> "sheila o'shea" <[log in to unmask]>
>
> Many thanks.
>
> Barbara Foster
> Microscopy/Microscopy Education
> 125 Paridon Street, Suite 102
> Springfield, MA 01118
> PH: 413-746-6931  FX: 413-746-9311  Web: www.MicroscopyEducation.com
>
> At 11:45 AM 6/12/02 +0100, you wrote:
> >Dear Barbara,
> >Do you know of any product (preferably mechanical) that is used  to keep
> >floating tissue in place on a glass bottom dish for  confocal imaging ? I
> >am trying to observe lymphatic tissue that has been treated with a
> >fluorescent probe.
> >Thank you for your time,
> >Regards,
> >Sheila
> >
> >
> >
> >Sheila O'Shea,
> >Department of Physiology,
> >Queens Medical Biology Centre,
> >97 Lisburn Road, Belfast
> >ext. 2073 or 07900200076
> >
> >
> >
> >
> >Sheila O'Shea,
> >Department of Physiology,
> >Queens Medical Biology Centre,
> >97 Lisburn Road, Belfast
> >ext. 2073 or 07900200076
> >
> >
> >_________________________________________________________________
> >Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.
> >

--
Russell McConnell
Confocal Imaging Facility Technician
Department of Neuroscience
Tufts University School of Medicine
M&V Building room #137
136 Harrison Ave.
Boston, MA 02111
Tel. (617) 636-3795
http://www.neurosci.tufts.edu/Imaging