Mime-Version: |
1.0 (Apple Message framework v546) |
Content-Type: |
text/plain; charset=UTF-8; format=flowed |
Date: |
Sat, 26 Oct 2002 14:22:26 -0400 |
Reply-To: |
|
Subject: |
|
From: |
|
In-Reply-To: |
|
Content-Transfer-Encoding: |
quoted-printable |
Sender: |
|
Parts/Attachments: |
|
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I have heard nothing more about this from my rep or otherwise. Are we
supposed to have gotten this software? Dave
On Saturday, May 4, 2002, at 12:53 AM, Martin Hoppe wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all,
>
> Some comments from a commercial source (Leica Microsystems) to
> Spectral Analysis, as requested by list users:
> --------------------------------------------------------------
> We announce the availability of new spectral analysis software for
> COnfocal Microscopy in the current month (May 2002).
> This software will work with all Leica TCS SP, SP2 and SP2 AOBS
> systems,
> including the high-speed RS (Resonant scanner) versions.
>
> The package completes the spectral scanning portfolio by
>
> Ã≈ xy-Lambda, xy-Lambda-t, xy-Lambda-z scans.
>
> Ã≈ Isolation of reference spectra from spectral scans.
>
> Ã≈ A spectra database with literature spectra. The database can be
> edited and items added during the experiment.
>
> The package contains the following multicolor restoration algorithms
> for crosstalk elimination:
>
> Ã≈ Spectral Dye Separation:
>
> transformation by using reference spectra known in advance or recorded
> calibration spectra.
>
> Ã≈ Adaptive Dye Separation:
>
> transformation with automatic identification of reference spectra from
> the image data set by statistical
> data analysis. This way, changes in the spectra during recording (e.g.
> due to environment, pH changes)
> are effectively detected and differences are reported to the user.
> This allows utilizing the advantages of the unmixing technology in a
> live cell environment.
>
> Ã≈ Manual Dye Separation:
>
> Manual (matrix based) separation
>
> Ã≈ The complete package works with full spectral recordings as well
> as with multi-channel image data,
> it does not necessarily require spectral scans.
>
> Ã≈ The output of the analysis tools can be analyzed additionally by
> the LCS ›Äì Multicolor option for co-localization studies.
>
> In this context, a basic benefit for obtaining clean primary spectra
> and image data are the new Leica TCS SP2 AOBS systems,
> utilizing a programmable acousto-optic multi-line beam splitter.
> This element improves collection efficiency over conventional beam
> splitters
> by 20-30% and it has a neutral characteristics with no spectral
> modulation in the visible spectrum.
> The combination of AOBS and optimized side window PMTÿs for detection
> allow imaging with low
> illumination intensity and minimum cell damage.
>
> Best regards
>
> Martin Hoppe
> ---------------------------------------------------
> Martin Hoppe, Ph.D.
> VP Marketing & Sales
> Leica Microsystems Heidelberg GmbH
> Am Friedensplatz 3
> D 68165 Mannheim / Germany
>
> Phone +49 (0)621-7028-0
> Cell phone: +49 (0) 172-623-0409
> Fax: + 49 (0) 621-7028-1180
> Email. [log in to unmask]
>
>
Dr. David Knecht
Department of Molecular and Cell Biology
U-125
75 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
|
|
|