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Does anyone know the 2-photon fluorescence cross section for a single
bacterium or spore for incident laser radiation around 450 nm - I believe
that the units would be GM = 10^-50 cm4sec.
I have the 2-photon cross sections for several fluorophores (rhodamine,
fluorescein, DiI, DAPI, Coumarin). They are in the range of 1/10 to 100
GM. If someone knows how 2-photon autofluorescence in bacteria compares
with any of these fluorophores, I can get an estimate of the bacterial
2-photon cross section. An order of magnitude estimate is adequate for my
present needs. Thanks for any assistance.
Lauren M. Peterson, PhD
Adj. Prof. Electr. Engin. and Comp. Sci.
1217 EECS (Bldg 2122)
Univ. of Michigan
Ann Arbor, MI 48109-2122
email: [log in to unmask] fax: 994-5704
(734)994-1200x2284 Veridian-ERIM